Adaptation of the MultiSite Gateway Pro 3-fragment recombination for use in bacteria.

Two Entry clones containing each a gene of interest (GOI) recombine (crossed grey lines) together with a third Entry clone and one Destination vector harboring each a reporter gene (R1/2) to create an Expression clone. The Expression clone contains three DNA elements donated from the three Entry clones and enables expression of the GOI1-R1 and GOI2-R2 fusion genes from tetracycline-inducible promoters (P_tetA). Transcription of fusion genes is stopped by a termination sequence derived from that of the rrnB operon (Ω).