Activities of pure TrCel5A and StCel5A on defined substrates.

Sigmacell is crystalline cellulose; PASC is phosphoric-acid swollen cellulose; lichenan and β-glucan are two types of mixed-linkage β1,3-β1,4 glucan, one from Iceland moss and the other from oats; xyloglucan is β1,4-linked glucan with α1,6-linked Xyl side chains from tamarind; mannan is insoluble β1,4-mannan; locust bean gum and guar gum are two types of β1,4-linked mannan with side chains of α1,6-linked galactose; xylan is β1,4-linked xylan; arabinoxylan is β1,4-linked xylan with α1,6-linked arabinose side chains; laminarin is predominantly β1,3-linked glucan. Shaded bars, StCel5A; open bars, TrCel5A. TrCel5A had no detectable activity against mannan, either galactomannan, xylan, or arabinoxylan. Neither enzyme had detectable activity against xyloglucan.