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Activation of p53 and Trp53-dependent proliferation arrest in Zfp148gt/gt MEFs.

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posted on 06.02.2013, 01:21 by Volkan I. Sayin, Anna Nilton, Mohamed X. Ibrahim, Pia Ågren, Erik Larsson, Marleen M. Petit, Lillemor Mattsson Hultén, Marcus Ståhlman, Bengt R. Johansson, Martin O. Bergo, Per Lindahl

(A) Western blots of MEF lysates showing expression of phospho-p53Ser18 in wt and Zfp148gt/gt MEFs cultured at 21 or 3% oxygen, respectively. Trp53−/− cells were used as a negative control and actin was used as a loading control. Asterisk indicates unspecific band. (B) Real-time RT-PCR of p21 in wt and Zfp148gt/gt MEFs cultured at 21 or 3% oxygen, respectively, and on Trp53+/+, Trp53+/− and Trp53−/− genetic backgrounds (n = 4). (C, D) CPD of Zfp148gt/gt and wt MEFs on Trp53+/+ or Trp53−/− (C) and Trp53+/+ or Trp53+/− (D) genetic backgrounds (n = 3). (E, F) CPD of Zfp148gt/gt and wt MEFs supplemented with n-acetyl-L-cysteine (NAC) (E) in the culture medium (n = 4), or cultured at atmospheric (21%) or low (3%) oxygen concentrations (F) (n = 3). *P<0.05, **P<0.01, ***P<0.001.

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