Activation of OVA-specific B3Z T-cells by T. gondii expressing OVA antigen.

Bone marrow derived macrophages (A) and dendritic cells (B) were stimulated with OT-I peptide or infected with T. gondii expressing OVA in various subcellular compartments (Fig. 1), and co-cultivated with B3Z T-cells in medium containing the ß-galactosidase substrate CPRG. Absorbance at 565 nm is represented relative to controls treated with media alone (average of three replicate assays). Results shown are representative of three independent experiments. Asterisks indicate p<0.05 (*) or p<0.005 (**), based on the students T-test statistic.