Figure_2.tif (56.66 kB)
Absolute quantification of intracellular iron content in monocyte subsets by Zeeman atomic emission absorption spectrometry.
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posted on 2013-02-20, 14:09 authored by Marcus Settles, Martin Etzrodt, Katja Kosanke, Matthias Schiemann, Alexander Zimmermann, Reinhard Meier, Rickmer Braren, Armin Huber, Ernst J. Rummeny, Ralph Weissleder, Filip K. Swirski, Moritz WildgruberFACS sorted monocyte subsets were incubated with four different iron-oxide nanoparticles (Ferumoxides, SHU 555C, CLIO-680, MION-48) at 100 µg Fe/ml for 2 h. Differences in intracellular iron contents between the subsets were assessed by ANOVA. P-values indicate significant differences in intracellular iron-content between the monocyte subsets. n = 3-5 for each bar graph. Iron levels of non-labeled control monocytes were below the detection limit.
