Ablation of PTP1B increases proliferation in in vitro transfected MIN6 cells and dispersed islet cells.
A) mRNA expression and B) protein content are used to confirm the efficiency of PTP1B siRNA transfection and silencing in MIN6 cells. Values are expressed relative to 36B4 as a housekeeping gene and actin protein content respectively. C) In vitro BrdU incorporation in proliferating si-ptpn1 MIN6 cells. Represented values are normalized to scrambled siRNA (Sc) MIN6 cells; n = 3 different experiments, 10 replicates per group each experiment. D) In vitro BrdU incorporation in PTP1B −/− proliferating dispersed islet cells is expressed relative to WT; n = 3 different experiments, 6 replicates per group each experiment. All bars represent mean±SEM * p<0.05, ** p<0.005 PTP1B −/− vs WT.