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A. baumannii OMVs interact with plasma membrane of host cells through a cholesterol-rich membrane microdomain.

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posted on 28.02.2011, 01:25 by Jong Sook Jin, Sang-Oh Kwon, Dong Chan Moon, Mamata Gurung, Jung Hwa Lee, Seung Il Kim, Je Chul Lee

(A) HeLa cells were treated with OMVs (5 µg/ml of protein concentrations) for 4 h. (B) HeLa cells were preterated with 10 mM MβCD for 45 min and then treated with OMVs for 4 h. The cells were fixed, permeabilized, and stained with anti-rabbit AbOmpA antibody, followed by Alexa Fluor® 488-conjugated rabbit IgG (green). DAPI was used to stain the nuclei (blue). Magnification: ×630.

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