(A) FACS analysis of peripheral blood against Mac3.

Significant increase in the Mac3 positive cells (R2) in Ang II-treated animals compared with control (R1); (B) FACS analysis of peripheral blood against CD45.2. Slight increase in the percentage of CD45.2 cells is seen in the Ang II-treated (R1) animals compared with control (R1); (C–E) FACS analysis of peripheral blood against Mac3. α-tocopherol-treatment slightly decreased Mac3 expression whereas no marked difference is seen in the β-carotene-treated group. Combined treatment shows downregulation of Mac3 on macrophages; (F–H) FACS analysis of peripheral blood against CD45.2. No marked difference is seen between the control and antioxidant-treated groups with CD45.2 expression.