Aβ is deposited at the Bruch's membrane (BM)/RPE interface and among photoreceptor outer segments.

A. The accumulation of amyloid beta in sections showing BM/RPE interface and the regions of the outer segments (OS) in mice of 3, 6 and 12 months age. Here Aβ label is red and the outer nuclear layer (ONL) is blue. This progressive accumulation was quantified with two independent methods at the two sites. First, the integrated density of label from immunostained sections was measured. The results of this are shown graphically in B and C. There are significant increases at both sites, particularly at 12 months (see text for levels of significance). Second, Western blots were run for Aβ at each site shown in D and E. F and G show the measurements at the same three time points as in B and C. The amount of Aβ increases significantly over time (see text for levels of significance). Differences between B and C and F and G are probably due to the different amounts of tissue sampled as F and G will also include measures derived from inner and outer retinal blood vessels.