AVEXIS reveals novel interactions involving P. vivax recombinant proteins.

(A) Heat map of the initial P. vivax intra-library AVEXIS, with the intensity of absorbance (A) values at 485 nm and positive putative interactions in red: P12-P41 (bait-prey and prey-bait orientations), P12-PVX_110945 (bait-prey orientation), and MSP3.10-MSP7.1 (bait-prey orientation). (*) indicates baits with low protein levels (< 0.5 μg/ml after concentrating) and preys with activity below the threshold required by the assay. Positive control (+ve) is the P. falciparum P12-P41 interaction. Negative controls (–ve) are rat Cd4d3+d4 tag in (A-C). (B) P12-P41 interaction within and between P. vivax (Pv) and P. falciparum (Pf) proteins by AVEXIS. An interaction between Pv P12-Pv P41 and Pv P12-Pf P41 in both bait and prey orientations. A, absorbance in (B-C). Bar chart shows mean with range; n = 2 in (B-C). (*) indicates n = 1. (C) Replicated P. vivax intra-library AVEXIS using re-synthesized PVX_110945 and MSP7.1 bait proteins, confirming the P12-PVX_110945 interactions in both orientations, and the MSP3.10-MSP7.1 interaction in only the bait-prey orientation.