ATP release from marginal cells.

A. Bafilomycin A1, a specific inhibitor of vacuolar-type H⁺-ATPase, inhibited the release of ATP from marginal cells. B. DDA, a specific inhibitor of adenylate cyclase, increased the release of ATP from marginal cells. C. Extracellular K⁺ concentration increased the release of ATP from marginal cells. D. Influence of thapsigargin, which decreases the intracellular Ca²⁺ concentrations inhibited the release of ATP from marginal cells. E. Extracellular Ca²⁺ concentrations inhibited the release of ATP from marginal cells. F. U73122 which affects the release of Ca²⁺ from intracellular stores inhibited the ATP releasing from marginal cells. G. Aristolochic acid, a PLA₂ inhibitor decreased the release of ATP from marginal cells. The release of ATP was significantly changed after each treatment (n = 8, P<0.05).