ASIC currents are present in NG2 cells.

(A) Top, schematics of whole-cell (left) and nucleated patch (right) recordings. Middle left, puffer application of acid onto an NG2 cell elicited inward currents (V-clamp at −80 mV) or membrane depolarization (I-clamp, V_m  = −80 mV before the pulse). Middle right, ASIC currents evoked by fast application technique in a nucleated patch from an NG2 cell (V-clamp at −60 mV). Bottom, summary of acid-evoked ASIC currents. (B) ASIC current density in NG2 cells compared to various types of neurons in the CA1 area and dentate gyrus. PN, pyramidal neuron; GC, granule cell; BC, basket cell; O-LM cell, oriens lacunosum-moleculare cell. **P<0.005; ***P<0.0005. (C) Top, ASIC currents evoked by different pH reductions. Bottom, the normalized peak amplitude of ASIC current plotted against the pH value. Data fit with the Hill equation. (D) Top, overlay of ASIC currents recorded at different potentials (−80, 0, +80 and +100 mV). Bottom, the I-V relationship had the reversal potential of +85 mV. Data (pooled from 8 cells) fit with a polynomial function.