AR-18 sensitizes PANC-1 xenografts to TRAIL.

(A) Schematic of in vivo treatment protocol. (B) β-catenin expression levels were quantified using densitometry and the resulting values were normalized against α-tubulin. Student’s t-test indicated that combination vs control: p = 0.0012, and for AR-18 vs control: p<0.0001. (C) Quantification of apoptosis in the tumor samples using H- score. Cleaved caspase-3 stained slides (as shown in Figure S2) were scored for intensity of the staining (I) as well as percentage of cells positive for the corresponding stain (H = I×P). Results of unpaired student t-test (n = 5 in each treatment group) are as follows: TRAIL vs control (p = 0.0002), combination vs control (p = 0.0004), combination vs TRAIL (p = 0.0261), combination vs AR-18 (p = 0.0035). (D) Evaluation of animal weight during treatment.