APP, CD45, and CD68 immunoreactivity increased in the visceral (pericardial) fat from high fat versus control diet fed mice with no robust change in Aβ immunoreactivity.

C57BL6/J mice at 6 weeks of age and weight matched were fed, ad libitum, a control (5.5% fat/weight) or high fat (21.2% fat/weight) diet for 22 weeks. Visceral pericardial adipose tissue was collected, immersion fixed, sectioned, and immunostained using anti-APP, Aβ, CD45 and CD68 antibodies and antibody binding visualized using Vector VIP as the chromogen. Representative images from 12 animals per condition are shown.