ALDH3A1 heterologous expression through the pET-26b(+) expression system.
figure
posted on 2013-02-23, 11:11 authored by Georgia-Persephoni Voulgaridou, Theodora Mantso, Katerina Chlichlia, Mihalis I. Panayiotidis, Aglaia PappaInduction at (A) 37°C, (B) 25°C and (C) 18°C. Samples were subjected to SDS-PAGE and stained with Coomassie blue. T0: Total cell extract form bacterial culture prior of protein induction, T3: Total cell extract 3 hours after induction, T6: Total cell extract 6 hours after induction, CE: Crude extract of lysed cells 6 hours after induction, IM: Insoluble matter of lysed cells 6 hours after induction. The arrow indicates the position of ALDH3A1/6xHis protein.
