ALDH3A1 heterologous expression through autoinduction with the pET-26b(+) expression system.
figure
posted on 2013-02-23, 11:11 authored by Georgia-Persephoni Voulgaridou, Theodora Mantso, Katerina Chlichlia, Mihalis I. Panayiotidis, Aglaia PappaSamples were subjected to SDS-PAGE and stained with Coomassie blue. 37°C, 25°C and 18°C: different induction temperatures, CE: Crude extract of lysed cells 9 hours after inoculation, IM: Insoluble matter of lysed cells 9 hours after inoculation. The arrow indicates the position of ALDH3A1/6xHis protein.
