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σ54 binds conserved sites in Salmonella enterica.

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posted on 01.10.2015, 02:52 by Richard P. Bonocora, Carol Smith, Pascal Lapierre, Joseph T. Wade

Targeted validation of predicted σ54 binding sites in S. enterica. Enrichment (occupancy units) at predicted σ54 binding sites in S. enterica was measured by ChIP-qPCR with anti-σ54 (black bars) or no antibody (white bars). The cognate promoter IDs (Tables 1 & 2) are indicated in parentheses. Error bars represent the standard deviation from three independent biological replicates. Significant differences between wild-type and ΔrpoN values are indicated (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001). Note that the y-axis scale differs for first five regions indicated. Underlined genes indicate σ54 binding sites previously identified by [14].