ppat.1008473.g005.tif (2.24 MB)

ZBP1 does not restrict the spread of VZV or VZV-RHIMmut in RIPK3-deficient, apoptosis-capable cells.

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posted on 10.07.2020, 17:39 by Megan Steain, Max O. D. G. Baker, Chi L. L. Pham, Nirukshan Shanmugam, Yann Gambin, Emma Sierecki, Brian P. McSharry, Selmir Avdic, Barry Slobedman, Margaret Sunde, Allison Abendroth

Empty vector or ZBP1 expressing ARPE-19s were inoculated with parental ARPE-19 cells infected with parent VZV (pOKA) or VZV-RHIMmut. z-VAD-fmk (25 μM) was added at the time of inoculation where indicated. After 72 h cells were fixed and immunostained for VZV IE63 (red) to assess virus spread. Images are representative from at least 2 independent replicates. Scale bars represent 400 μm.