Yeast-two-hybrid experiments testing interactions between Arabidopsis FIGL1, FLIP, RAD51 and DMC1 proteins.

Proteins of interest were fused with Gal4 DNA binding domain (BD, left) and with Gal4 activation domain (AD, top), respectively, and co-expressed in yeast cells. Full-length and truncated protein are schematically represented. For each combination, serial dilutions of yeast cells were spotted on non-selective medium (-LW), moderately selective media (-LWH) and more selective media (-LWHA). ++: Growth on both LWH and LWHA, interpreted as strong interaction. +: Growth on LWH and not on LWHA, interpreted as weak interaction. +*: Growth on LWH but cannot be interpreted as positive interaction because of auto-activation of one of the construct.—: Growth on neither LWH nor LWHA. n.d. Not determined. Pictures of yeasts are shown in S1 Fig.