pone.0225658.g006.tif (1.51 MB)
Volcano plots of immune-related genes expressed in tissues collected from chickens raised on pedigree and sibling test farms.
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posted on 2019-12-03, 18:44 authored by Dominika Borowska, Richard Kuo, Richard A. Bailey, Kellie A. Watson, Pete Kaiser, Lonneke Vervelde, Mark P. StevensScattered points represent genes; the x-axis is the log2 fold change for the ratio sibling test vs pedigree farm, whereas y-axis is the log10 p-value.
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multiplexed qPCR 96.96 Fluidigm Dynamic Array6 nanolitre reactionsbroiler chickensPCR-based platformmicrofluidic system96 transcriptstranscript levelstranscriptome dataamplicon sequencingchicken macrophagesRNA-seq analysispreamplification stepsConventional qPCR analysisGut-associated lymphoid tissuesblood cellstranscriptional response89 genesimmune-related genesbiosecurity levelsmultiplexed qPCR-based platformscreen vaccine responseschicken immune-related genesmultiplex qPCR array96 samplesanalysehigh-throughput assaysblood leukocytescurve analysispathogen-specific probesdendritic cells
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