Validation of the expression levels of 3 genes.

(A) The relative expression levels of 3 genes were normalized to that of β-actin. (B) Western blot analysis was used to validate the expression level of PLZF in the 3 groups. (C) Immunohistochemistry and semi-quantitative analysis for PLZF. The data were expressed as the mean ± SEM. #P<0.05, ###P<0.001 vs. db/m group; *P<0.05, **P<0.01 vs. db/db group.