Fig 6.TIF (237.06 kB)
Validation of the RNA-Seq results using quantitative real-time PCR.
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posted on 2016-10-17, 17:33 authored by Jian Huang, Lei Zhao, Ping Yang, Zhen Chen, Ni Tang, Xiong Z. Ruan, Yaxi ChenThe values are presented as the means±SD (n = 4). Comparisons among groups were determined with independent-sample t-test using the SPSS software (Version 11.5). Significant differences are considered at *P<0.05, ** p < 0.001 and *** p < 0.0001 versus control group.β-actin was used as the reference gene.
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CD 36gene ontologyCD 36 overexpressionCD 36OE cellsHepG 2.2.15 CellsCD 36 OverexpressionHBV life cycleHBV gene expressionHepG 2.2.15-CD overexpressionDEGgene expressionRecent studiesHCVHepatitis B Virus Life Cycle Hepatitis B virusHBV replicationqPCR analysisgenome-wide sequencinghepatocyte-specific DNA virusRegulatory RoleRNA-Seq datahepatitis C virus141 differentiallyHepG 2.2.15 cellsimmunodeficiency virusCD 36 expressionHepG 2.2.15-vector cellsacid translocase CD 36HIVKEGG pathway analysisGenome-Wide Transcriptome Analysis62 downregulated genesRNA Sequencing79 upregulated genestranscriptomic profile
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