Validation of RNA-seq results by RT-qPCR with GAPDH as the endogenous control.
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posted on 2020-09-24, 17:29 authored by Hehe Tang, Yaping Guo, Zhenzhen Zhang, Zhuanjian Li, Yanhua Zhang, Yuanfang Li, Xiangtao Kang, Ruili HanExpression level was calculated using the comparative cycle threshold (2-ΔΔCt) value method. The data were expressed as the mean±SEM (*P < 0.05; **P < 0.01).
