Validation of MIND model and detection of different receptors’ status in various human and mouse breast cancer cell lines.

(A) Schematic representation of mouse mammary intraductal injection through cleaved nipples. Mvt-1 or 4T1 cells were re-suspended in 0.04% trypan blue and injected through 4^th inguinal mammary gland cleaved nipple. The experimental protocols are depicted in the left two panels. (B) Representative images of α-SMA and laminin-1 immunostaining in human biopsy samples (n = 26) and MCF-DCIS-MIND xenograft samples (n = 5). The images illustrate the similarity of the Human breast and MIND xenograft architecture. White arrows indicate staining (green) of laminin and α-SMA and Red arrows indicate possible localized death of MECs. Scale bar, 50μm. (C) Representative Western blot analysis of ER-α, PR and HER2 expressions in the cellular extracts of SKBR3, MCF-7, MDA-MB-231, Mvt-1, and 4T1 cell lines. SKBR3 cell extract was used as positive control for HER2, and MCF-7 cell extract was used as positive control for ER-α and PR. (n = three replicates). All photographs are cropped from original figures.