pone.0225658.g007.tif (670.39 kB)
Validation of 96.96 Dynamic Array qPCR results for selected 3 transcripts: TNFAIP3, SAAL1, and IRG1.
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posted on 2019-12-03, 18:44 authored by Dominika Borowska, Richard Kuo, Richard A. Bailey, Kellie A. Watson, Pete Kaiser, Lonneke Vervelde, Mark P. StevensRelative expression of three genes tested between sibling-test and pedigree farm in 96.96 Dynamic Array, and in conventional 96-well plate format qPCR Applied Biosystems 7500 Fast Real-Time PCR System; each bar represent average of biological replicates from 4 birds and 5 tissues; error bars depict SEM, **—P-value ≤ 0.0005; *—P-value ≤ 0.05.
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multiplexed qPCR 96.96 Fluidigm Dynamic Array6 nanolitre reactionsbroiler chickensPCR-based platformmicrofluidic system96 transcriptstranscript levelstranscriptome dataamplicon sequencingchicken macrophagesRNA-seq analysispreamplification stepsConventional qPCR analysisGut-associated lymphoid tissuesblood cellstranscriptional response89 genesimmune-related genesbiosecurity levelsmultiplexed qPCR-based platformscreen vaccine responseschicken immune-related genesmultiplex qPCR array96 samplesanalysehigh-throughput assaysblood leukocytescurve analysispathogen-specific probesdendritic cells
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