pone.0225658.g002.tif (2.44 MB)
Validation of 96.96 Dynamic Array.
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posted on 2019-12-03, 18:44 authored by Dominika Borowska, Richard Kuo, Richard A. Bailey, Kellie A. Watson, Pete Kaiser, Lonneke Vervelde, Mark P. StevensA) standard RNA samples of COS-7 cells transfected with plasmids containing genes for: CXCLi2, IL1B, IL12B, IL6, IL18, reverse transcribed and used in limiting dilutions; B) cDNA and cDNA after preamplification showing higher amplification in the later; brighter colour indicates lower Cq value, higher amplification.
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multiplexed qPCR 96.96 Fluidigm Dynamic Array6 nanolitre reactionsbroiler chickensPCR-based platformmicrofluidic system96 transcriptstranscript levelstranscriptome dataamplicon sequencingchicken macrophagesRNA-seq analysispreamplification stepsConventional qPCR analysisGut-associated lymphoid tissuesblood cellstranscriptional response89 genesimmune-related genesbiosecurity levelsmultiplexed qPCR-based platformscreen vaccine responseschicken immune-related genesmultiplex qPCR array96 samplesanalysehigh-throughput assaysblood leukocytescurve analysispathogen-specific probesdendritic cells
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