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U-ExM confirms the organisation of the actin cytoskeleton during P. falciparum gametocytogenesis.

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posted on 2022-01-25, 18:43 authored by Ravish Rashpa, Mathieu Brochet

A-D. Representative full projections of P. falciparum gametocyte stages. α/β tubulin: magenta; amine reactive groups / NHS-ester: shades of grey; actin: cyan. Column 6 shows the 3D surface topology reconstruction of α/β-tubulin and actin. A and B. At early stages, a punctate distribution of actin is observed. C-D. From stage IV gametocytes, actin shows a polarised localisation, mostly concentrated at both extremities in tubulin-free areas showing a mesh-like organization. Actin also extends lengthwise alongside the subpellicular microtubules. Insets in (C and D) are close ups of both extremities. Scale bars = 5 μm.