Two sample comparison of the CD62p and CD41 distributions in clots.

(a) and (b) show reconstructed 3D dSTORM images (approx. 100 000 data points/image). (a) shows the 3D distribution of CD41 (Alexa488-antibody). (b) represents the 3D distribution of CD62p (Alexa647-antibody). (c) shows the comparison of the cluster densities for all given cluster dimensions between the two datasets (1^st-level comparison). The blue/red lines depict the KS- and WX-test results, respectively. The aggregated p-value for KS and WX tests (dashed black line) remains within the critical p-value-area (orange) disproving the similarity hypothesis. The zoomed-in areas depict: a cluster dimension (5 nm—200 nm, sim_M, sim_L below 0.5); pairwise KS- and WX-test comparisons of cluster densities for both samples indicate dissimilarity (left) and a second cluster dimension (800 nm—1000 nm, sim_M, sim_L larger 0.5); pairwise KS- and WX-test comparisons of cluster densities for both samples show similarity (right). (d) shows the 2^nd-level comparison of the mean cluster density for all given cluster dimensions. The mean-cross p-value comparison (blue) and lower/upper confidential bounds (grey dashed line) are shown. (e) and (f) represents 600 clusters localized within the CD41/CD62p distribution respectively, displayed using the Delaunay-triangulation method (clustering dimension 390 nm). (g) shows two representative clusters from from (e) and (h) from (f).