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Download fileTwo-dimensional 1H-15N HSQC spectrum of L50E and L51E.
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posted on 05.08.2022, 20:04 authored by Fei Tan, Jin XuRibbon representation of the (a) solution structure (gold) and (b) crystal structure (cyan) with the side chains L50 and L51 in orange and green while the side chains of the residues interacting with L50 or L51 shown in blue and mud yellow for solution and crystal structures respectively. (c) Two-dimensional 1H-15N HSQC spectra of L50E. (d) Two-dimensional 1H-15N HSQC spectra of L51E, residues experiencing great chemical shift changes (Δ comp ≥ 0.3 ppm) were labeled.
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hydrophobic core packingdeuterium exchange experiments>- helix h2proteins called microtubulecycle dependent proteinsn terminal residuesalso generated mutantsdiv >< pconformation prc1 adoptedhigh resolution threeprc1 </ presolution solution structureassociated proteinsn terminusalso differentalpha </prc1 ’prc1 callswhole proteinused hydrogenthermal stabilityspindle midbodysolution structuressolution structureresults suggestplacing hurdlesphysiological significancenmr spectroscopyincomplete sidechainshuman mapfunction relationshipsdistinguishing memberdimerization domaindimensional structurebind microtubules
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