Transient CRISPR-cas9 cloning diagrams.
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posted on 2025-03-17, 17:35 authored by Melissa A. Tosiano, Frederick Lanni, Aaron P. Mitchell, C. Joel McManusMethods for A. inserting a fluorescent tag in the genome, B. deleting the entire ORF for a gene leaving only a 34 bp FRT scar, and C. generating a DHH1 complement at the MDR1 site. Diagram produced using Biorender.
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previous work reportedhuman fungal pathogenfive strain backgroundshyphal transcriptional networkshyphal growth conditionsgenerated homozygous deletionsdiv >< pcandida albicans cerevisiae dhh1 hyphal growthalbicans dhh1 factors dhh1using rnatranscriptional regulationstrongly associatedresults supportrelatively littleincluded upregulationheat toleranceheat stressexternal stressessential rolediverse strainsbolstering evidence
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