Tight junctions remain present during migration and delamination stages.
Representative images of Tg(fli1a:LifeAct-EGFP) embryos immunostained with ZO-1 at 60, 65, and 80 hpf. The rightmost column shows the ZO-1 signal overlaid on top of an image showing our interpretation of valve cell morphology based on the EGFP signal. Dotted lines outline the Myo. Scale bar: 10 μm. hpf, hours postfertilization; Myo, myocardium; ZO-1, zonula occludens-1.