Thermocycling-induced DNA damage.
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posted on 2017-01-06, 19:20 authored by Vladimir Potapov, Jennifer L. OngThe base substitution error rates of plasmid libraries without treatment, mock thermocycling, and mock thermocycling and PreCR treatment. The base substitution error rate of plasmid libraries treated with PreCR before and after SMRTbell library construction represents the background error rate of the single-molecule sequencing fidelity assay.
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single-molecule levelPolymerase Chain ReactionPCR-mediated recombinationnext-generation sequencing technologiesmutationsingle-molecule sequencing assaymultiplex amplification reactionsDNA amplificationsample preparation workflowsDNA damagewell-characterized polymerase base substitution errorspolymerase base substitution errorsSingle-Molecule Sequencing Next-generation sequencing technology
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