The topography of rods, cones and intrinsically photosensitive retinal ganglion cells in the retinas of a nocturnal (Micaelamys namaquensis) and a diurnal (Rhabdomys pumilio) rodent - Fig 2

Immunolabelling of melanopsin positive ganglion cells in Micaelamys namaquensis (A, C) and Rhabdomys pumilio (B, D). (A, B) Flattened retinae showing ipRGCs dendritic arbors with beaded varicosities. The upper right insets show putative M2 type ipRGCs, which were faintly labelled and had larger somata. (C, D) Vertical sections of the retina demonstrating that the somata of ipRGCs are situated in the GCL with dendrites stratifying in the outermost margin (OFF sublamina) of the IPL. GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer; R/C, outer segments of rods and cones. The binding sites of the primary antibodies were visualized by a peroxidase anti-peroxidase reaction (diaminobenzidine staining) in (A–C) and by Alexa 488 conjugated secondary antibody in (D). Scale bars; 100 μm.