The Poz1-binding domain and BRCT domain of Rap1 redundantly suppress GCRs.
(A) Schematic of Rap1 truncation mutants. (B) GCR rates of the Rap1 mutants shown in (A). Rap1 proteins were expressed from a single copy of cognate alleles integrated downstream of the native rap1 promoter. FL, full length. Asterisks represent significant differences (P < 0.05) from the wild type strain. P-values were obtained by the two-tailed Mann–Whitney test. (C) Numbers of different types of GCRs identified in 5-FOA/FUdR-resistant clones derived from wild-type strain.