TLC analysis for lipids from the stratum corneum.

TLC of SC lipids from 3DHEEs models treated with (A) SSE and (B) tiliroside. For measurement of SC ceramide (Cer), the lipid samples were developed twice on a TLC plate. Namely, a mixture of chloroform, methanol, and acetic acid (190:9:1) was used for the first development, and mixture of chloroform, methanol, and acetic acid (197:2:1) was used for the second development. The spots for ceramide were visualized using 10% copper sulfate in 8% phosphoric acid aqueous solution.