Summary of the modifications to the slot blot assay protocol.
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posted on 2017-04-21, 17:23 authored by Bryan Grabias, Nitin Verma, Hong Zheng, Abhai K. Tripathi, Godfree Mlambo, Merribeth J. Morin, Emily Locke, Sanjai KumarA number of changes were implemented to improve overall assay performance, reduce assay time, and minimize reagent and film requirements to better allow broad usage of the ECL-SB in resource poor field sites. The time required to perform the slot blot has been reduced by more than half of the original protocol length and the sensitivity for Pfoocysts has been improved from to 0.25 oocyst to 0.02 oocyst.
