pone.0200673.g008.tif (356.55 kB)
Summary of main findings from chIP analysis of AbsA2 binding.
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posted on 2019-04-10, 17:29 authored by Richard A. Lewis, Abdul Wahab, Giselda Bucca, Emma E. Laing, Carla S. Möller-Levet, Andrzej Kierzek, Colin P. SmithSchematic diagram illustrating the in vivo pattern of binding of 3xFabsA2 to selected genes from antibiotic biosynthetic gene clusters known, or predicted, to be bound by AbsA2. References providing evidence of AbsA2 binding are indicated alongside those chIP peaks they provide supporting evidence for. ‘Biphasic’ chIP peaks are shown in purple whilst ‘monophasic’ chIP peaks are shown in blue.
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actII-orfIV AbsA 2antibiotic phenotypic assaysN-terminal triple-FLAG-tagged versionSCOabsA 2 geneAbsA 2 bindingvivo AbsA 2 DNA-binding target sitesmicroarray-based time-course experimentsantibiotic biosynthetic gene clustersStreptomyces coelicolorcda biosynthetic gene clusterCDA biosynthetic genesantibiotic biosynthesis regulator AbsA 2antibiotic hyper-production phenotype
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