Study of cell fate determinants of Apterous neuron specification in Mcm5 mutants.
(A-B) Expression of the temporal factors Castor (Cas) and Grainy head (Grh) in the Ap cluster in control and Mcm5 mutants, at St16. Eya was used to visualize Ap neurons. (E-J) Expression of Ap neuron determinants Nab, Squeeze (Sqz), Dimmed (Dimm) and phosphorylated form of Mothers against dpp (pMad) in control and Mcm5 mutants, at stage 18 AEL. Eya was used to visualize Ap neurons. (K-N) Expression of ap based on the ap-Gal4>UAS-GFP reporter expression, Dachshund (Dac) and Seven up (Svp) in control and Mcm5 mutants, at stage AFT. Merge and individual antibody images are shown in each panel. Representative images of n≥3 CNS per genotype, n≥ 18 hemisegments per genotype. Genotypes: (A, C, E, G, I, K, M) w1118. (B, D, F, H, J, L, N) Mcm5exc222/Mcm5exc222 (Scale bar 5μm).