Structural details of TbAQP2 chimerisation and selectivity filters.

3D structural predictions of N-terminal tagged TbAQP1 (cyan), TbAQP2 (magenta), TbAQP3 (green) and the chimeric TbAQP2/3 40AT and AQP2^TMD4, with the corresponding domain swap colour-coded. Structures were calculated with i-Tasser. The 3xHA-tag is omitted for simplicity. Details of the selectivity pore for each of these proteins (12Å) are also included. For TbAQP2, AQP2^TMD4 and 40AT constructs, the selectivity filter is composed of the “NSA/NPS” motif, whereas TbAQP1 and TbAQP3 contain a “NPA/NPA” motif.

(AI)