Statistical analysis of NRC ZAR1 α1 helix chimaera complementation of Cf-4/Avr4-triggered cell death N. benthamiana nrc2/3/4 CRISPR lines.
A) Statistical analysis was conducted using besthr R package . The dots represent the ranked data and their corresponding means (dashed lines), with the size of each dot proportional to the number of observations for each specific value (count key below each panel). The panels on the right show the distribution of 100 bootstrap sample rank means, where the blue areas under the curve illustrate the 0.025 and 0.975 percentiles of the distribution. A difference is considered significant if the ranked mean for a given condition falls within or beyond the blue percentile of the mean distribution of the wild-type control. B) NRC ZAR1 α1 helix chimaeras can complement Cf-4/Avr4 and Pto/AvrPto-triggered hypersensitive cell death in the N. benthamiana nrc2/3/4 CRISPR lines. Representative N. benthamiana leaves infiltrated with appropriate constructs were photographed 7–10 days after infiltration. The receptor/effector pair tested, Cf-4/Avr4 and Prf (Pto/AvrPto), are labelled above the leaf of NRC CRISPR line nrc2/3/4-18.104.22.168. The NRCs tested, NRC2 and NRC3, are labelled on the leaf image. To ensure the NRC ZAR1 α1 helix chimaeras were not autoactive they were expressed with either Cf-4 or Pto and an EV control was taken along.