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Statistical analysis of Cf-4/Avr4-suppression mediated by divergent pathogen effectors.

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posted on 22.09.2022, 18:17 authored by Jiorgos Kourelis, Mauricio P. Contreras, Adeline Harant, Hsuan Pai, Daniel Lüdke, Hiroaki Adachi, Lida Derevnina, Chih-Hang Wu, Sophien Kamoun

A) Statistical analysis was conducted using besthr R library [72]. The dots represent the ranked data and their corresponding means (dashed lines), with the size of each dot proportional to the number of observations for each specific value (count key below each panel). The panels on the right show the distribution of 100 bootstrap sample rank means, where the blue areas under the curve illustrates the 0.025 and 0.975 percentiles of the distribution. A difference is considered significant if the ranked mean for a given condition falls within or beyond the blue percentile of the mean distribution of the wild-type control. B) Cf-4/Avr4-triggered hypersensitive cell death is suppressed by SPRYSEC15 and AVRcap1b, but not the EV control. Representative image of N. benthamiana nrc2/3/4- CRISPR line leaves which were agroinfiltrated with NRC3/suppressor constructs, as indicated above the leaf, and either autoactive NRC3D480V, Prf (Pto/AvrPto), R3a/Avr3a, or Cf-4/Avr4 as labelled on the leaf image, photographed 7–10 days after infiltration.