Fig 2.TIF (1.1 MB)
Southern blot hybridization results.
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posted on 2016-10-06, 17:39 authored by Yanzheng Song, Fengju Zhang, Yanyan Zhao, Mingshen Sun, Jun Tao, Yanchuang Liang, Ling Ma, Yanqiu Yu, Jianhua Wang, Junfeng HaoThe genomic DNA extracted from six transgenic mice and one wild-type mouse was digested with EcoR I and Bgl II and hybridized using a DIGD-110-labeled probe. Lane 1 to lane 6, EcoR I- and Bgl II-digested DNA from six mouse tails (No. 9, No. 19, No. 20, No. 22, No. 24, and No. 31). Lane 7, genomic DNA fragments from the wild-type mouse as a negative control. Lane 8 to lane 9, PCR products of transgene LUM as positive controls.
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Altered Ultrastructural Featureswild-type B 6 micescleracollagen typeWestern blot analysisCOLultrastructuralcollagen fibrilscollagen fibril diameterMYP 3 locuslengthMutant Lumican Transgenic Mouse Model LumicanLUMgene expression levelsTEMpolymerase chain reactiontransmission electron microscopymyopiamouse modelL 199Pprotein levelslumican
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