Sister chromatid segregation in telomere clustering-defective cells.

(A) Chromosome segregation patterns at meiosis I in rec12Δ diploid zygotes. (B and C) The frequencies of equational segregation of sister chromatids at meiosis I in rec12Δ (B) and rec12Δ sgo1Δ (C) diploid zygotes. (D) Chromosome segregation patterns at meiosis I in haploid cells. (E and F) Effects of bqt1Δ mutation (E) or MBC treatment (F) on sister chromatid segregation in haploid meiotic cells. Sister chromatid segregation was analyzed by visualizing the centromere-proximal region of chromosome II [40]. +: no mutations otherwise depicted; DMSO: treated with DMSO; MBC: treated with MBC. Averages of three independent experiments are shown. Error bars indicate standard deviation. More than 60 cells were examined in each experiment. Lines indicate pairs of data that were statistically compared. *p<0.05; **p<0.005; ns: no significant difference (by the Student’s t-test).