The VP1 amino acids sequences of A/AF72, A/WH/CHA/09 and A/GDMM/2013 strains (marked with diamond) were aligned with that of 48 serotype A strains retrieved from NCBI, including sevens lineages from ASIA topotype, four lineages from EURO-SA topotype and four lineages from AFRICA topotype. The identified key determinations at positions 58 and 147 were circled with blue box and the strains with varied residues involving determinations were indicated with red circle.