Sarm1 transgene levels.

A. Sarm1-HA tagged transgenes were expressed under control of the dVGlut-Gal4 driver. The segmental nerves of third instar larvae are shown visualizing HA-tagged dSarm1 (anti-HA antibody: C29F4 1:1500 dilution). Images of transgenes from the same class (isoform E- 3KB or isoform D- 5KB) were taken at similar levels. B. Quantification of transgene levels. Quantification of images for all genotypes were taken at the same gain and intensity settings. Values are presented as mean +/- standard deviation (n = 4, 4, 4, 5, 5, 5, 4, 5, 5 for each genotype). Statistics obtained by performing a one-way ANOVA with Tukey’s multiple comparisons for genotypes (****p<0.0001). The mean intensity of fluorescence was quantified in image J.

(EPS)