Sampling locations for Aplocheilus panchax over 19 areas.

Tamil Nadu (TN), Kolkotta (KK), Cambodia (CB), Vietnam (VT), Krabi (KB), Sungai Batu Pahat (SBP), Aceh (AC), Penang (PN), Dungun (DG), Pulau Laut (PL), Singapore (SP), Pekanbaru (PK), West Sumatra (WS), Jambi (JB), Bogor (BG), Surabaya (SR), Banjarmasin (BJ), Bali (BL) and Sulawesi (SL). Points are coloured according to which of the three major mitochondrial clades they correspond to (see ‘Results‘, Fig 2), and stars for locations where nuclear loci were also sampled. Shaded areas indicate the four biodiversity hotspots in Southeast Asia: Sundaland, Wallacea, Philippines and Indo-Burma. Wallace’s Line, Huxley’s modification of Wallace’s Line (based on zoological data; [8]) and the Isthmus of Kra are demonstrated by the red dashed lines.