SIRT3 overexpression increased mitochondrial biogenesis in ccRCC cells.

a. SIRT3 mRNA and protein levels were assessed using qRT-PCR and western blotting after SIRT3 overexpression (C: empty vector, S: SIRT3 expression vector). Values are expressed as mean ± SEM (n = 3, per triplicate) and control-cell values were set to 1. b. qRT-PCR was used to assess the mRNA levels of PGC1α and PHD3 after SIRT3 overexpression. Values are expressed as mean ± SEM (n = 5, per triplicate) and control-cell values were set to 1. *p <0.05, **p <0.01. c. Western blotting was used to assess PGC1α and PHD3 protein levels after SIRT3 overexpression. Representative images from three replicates. Quantification of proteins ratio obtained from triplicate samples and normalized onto β-Actin and represented in the bars graph. *p <0.05, **p <0.01, ***p <0.001.