pntd.0009322.s009.tif (233.07 kB)
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posted on 2021-04-08, 18:02 authored by Ana K. Ibarrola-Vannucci, Luis M. De Pablos, Lissette Retana-Moreira, Alberto Cornet-Gomez, Teresa Cruz-Bustos, Alejandro G. Schijman, José L. Ramírez, Susana Vílchez, Antonio OsunaA. Schematic representation of the PCR strategy used to amplify the upstream and downstream regions of the PHB1 ORF. B. 1.5% Agarose gel showing the expected PCR products upstream (1) and downstream (2) the PHB1 locus in the PHB1 Clone 1 (CRISPR-Cas9 gene edited) and WT cell lines.
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bioinformatics analysisTrypanosoma cruzi Background Chagas...metacyclic trypomastigotescruzi clonesPHB 2PHB 2 parasitesside effectsnon-infective formsPHB 1 parasitesproteins Prohibitin 1ROSelectron microscopytransformation rateimmuno-electron microscopylife cyclePHB genespleiotropic functiontransfected Tintracellular multiplicationmetacyclic infective formsintracellular amastigotesCRISPRnon-infective replicative epimastig...cruzi PHBs
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