S8 Fig -

Agarose gel electrophoresis for DNA digests generated by CRISPR-Cas9 (A) or strandase (B). (A) Lane M: 100 bp DNA ladder; lane 1 & 3: linear mNeonGreen template DNA; lane 2: cleaved by Cas9-sgRNA targeting a site around BamHI, producing two DNA fragments about 180 bp and 820 bp; lane 4: cleaved by Cas9-sgRNA targeting another site around NcoI/BtgI, producing two DNA fragments about 440 bp and 560 bp. (B) Lane M: Hi-Lo DNA marker; lane 1: linear mNeonGreen template DNA phosphorylated at 5'-end of the antisense strand; lane 2: sense ssDNA; lane 3: linear mNeonGreen template DNA phosphorylated at 5'-end of the sense strand; lane 4: antisense ssDNA. In general, fluorogenic intercalators stains the ssDNA much less efficiently than for dsDNA. All lanes were loaded with 100 ng DNA sample.

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