posted on 2021-04-15, 18:01authored byYing Li, Han Gong, Rui Zhan, Shushan Ouyang, Kyung-Tae Park, Joe Lutkenhaus, Shishen Du
Overexpression of FtsIK211I (A) or FtsWE289G (B) tolerates the depletion of FtsN. Plasmid pDSW208, pLY91 (pDSW208, P204::ftsI), pSEB429 (pDSW208, P204::ftsW), or their derivatives carrying different ftsI or ftsW allele were transformed into strain SD264 [W3110, ftsN::kan /pBL154 (pSC101ts, ftsN)] and transformants selected on LB plates with antibiotics and glucose at 30°C. The test was performed as Fig 3B.